Skip to main content
Addgene

pGEXNKI-his-GST-3C-LIC
(Plasmid #108709)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 108709 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pGEX
  • Backbone manufacturer
    Novagen
  • Backbone size (bp) 5152
  • Vector type
    Bacterial Expression
  • Promoter tac
  • Tag / Fusion Protein
    • GST-6xHis-3C protease cleavage site (N terminal on insert)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEXNKI-his-GST-3C-LIC was a gift from Titia Sixma (Addgene plasmid # 108709 ; http://n2t.net/addgene:108709 ; RRID:Addgene_108709)

  • For your References section:

    Enabling high-throughput ligation-independent cloning and protein expression for the family of ubiquitin specific proteases. Luna-Vargas MP, Christodoulou E, Alfieri A, van Dijk WJ, Stadnik M, Hibbert RG, Sahtoe DD, Clerici M, Marco VD, Littler D, Celie PH, Sixma TK, Perrakis A. J Struct Biol. 2011 Aug;175(2):113-9. doi: 10.1016/j.jsb.2011.03.017. Epub 2011 Mar 29. 10.1016/j.jsb.2011.03.017 PubMed 21453775
Related items:
Commonly requested with: