pEGFP‐RNASEH1
(Plasmid #108699)

• Purpose: For expression of C-terminally eGFP-tagged human RNASEH1 (nuclear isoform) in mammalian cells
• Depositing Labs: Andrew Jackson, Martin Reijns
• Publication: Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-66. doi: 10.1093/nar/gkq980. Epub 2011 Jan 17.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4733
• Total vector size (bp): 5500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RNASEH1 (nuclear isoform)
• Species: H. sapiens (human)
• Entrez Gene: RNASEH1 (a.k.a. H1RNA, PEOB2, RNH1)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EGFP-N

Resource Information

• Supplemental Documents:
  • pEGFP-RNASEH1.gb
• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 12 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry