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Addgene

pRS316-VTC4
(Plasmid #108691)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 108691 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS316
  • Backbone size w/o insert (bp) 4887
  • Total vector size (bp) 7636
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Vacuolar Transporter Chaperone 4
  • Alt name
    VTC4
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2766
  • Promoter VTC4 (native promoter)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer several primers were used
  • 3′ sequencing primer several primers were used
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS316-VTC4 was a gift from Michael Downey (Addgene plasmid # 108691 ; http://n2t.net/addgene:108691 ; RRID:Addgene_108691)

  • For your References section:

    A Screen for Candidate Targets of Lysine Polyphosphorylation Uncovers a Conserved Network Implicated in Ribosome Biogenesis. Bentley-DeSousa A, Holinier C, Moteshareie H, Tseng YC, Kajjo S, Nwosu C, Amodeo GF, Bondy-Chorney E, Sai Y, Rudner A, Golshani A, Davey NE, Downey M. Cell Rep. 2018 Mar 27;22(13):3427-3439. doi: 10.1016/j.celrep.2018.02.104. 10.1016/j.celrep.2018.02.104 PubMed 29590613
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