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Addgene

CAA 659
(Plasmid #108684)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 108684 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC57-Simple
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CAA repeat mRNA
  • Species
    Synthetic
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer M13
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pUC plasmids do not have a T7 promoter, therefore it was included in the plasmid insert during cloning, 5' to the desired RNA sequence.

For In vitro transcription, the plasmid was cut with SspI-HF for the transcription to run off.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CAA 659 was a gift from Jon Lorsch (Addgene plasmid # 108684 ; http://n2t.net/addgene:108684 ; RRID:Addgene_108684)

  • For your References section:

    Yeast eIF4A enhances recruitment of mRNAs regardless of their structural complexity. Yourik P, Aitken CE, Zhou F, Gupta N, Hinnebusch AG, Lorsch JR. Elife. 2017 Nov 30;6. doi: 10.7554/eLife.31476. 10.7554/eLife.31476 PubMed 29192585
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