pVKG1-BSD
(Plasmid
#108680)
-
PurposeBlasticidin resistant cassette for knock-in by VIKING method
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 108680 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepENTR/D-TOPO
-
Backbone manufacturerInvitrogen
-
Selectable markersBlasticidin
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameSV40pro-BSD-polyA
-
Insert Size (bp)1750
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pVKG1-BSD was a gift from Shigeo Sugano (Addgene plasmid # 108680 ; http://n2t.net/addgene:108680 ; RRID:Addgene_108680) -
For your References section:
Development of versatile non-homologous end joining-based knock-in module for genome editing. Sawatsubashi S, Joko Y, Fukumoto S, Matsumoto T, Sugano SS. Sci Rep. 2018 Jan 12;8(1):593. doi: 10.1038/s41598-017-18911-9. 10.1038/s41598-017-18911-9 [pii] PubMed 29330493