p3186 HPV-16 E1
(Plasmid
#10868)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 10868 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX-4T1
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Backbone manufacturerAmersham
- Backbone size w/o insert (bp) 4969
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHPV-16 E1
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Alt nameE1
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SpeciesHuman papillomavirus
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GenBank IDNP_041327
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Entrez GeneE1
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer pGEX5' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
HPV-16 E1 (aa1-649) from Lex202PL-derived plasmid. See author's map.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p3186 HPV-16 E1 was a gift from Peter Howley (Addgene plasmid # 10868 ; http://n2t.net/addgene:10868 ; RRID:Addgene_10868) -
For your References section:
Mapping and characterization of the interaction domains of human papillomavirus type 16 E1 and E2 proteins. Yasugi T, Benson JD, Sakai H, Vidal M, Howley PM. J Virol. 1997 Feb . 71(2):891-9. PubMed 8995605
Map uploaded by the depositor.
