VKG1-gRNA-pX330
(Plasmid
#108671)
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PurposeCleavage of VKG1 sequence by CRISPR/Cas9
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 108671 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepX330
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Modifications to backboneVKG1-gRNA 5'-AACGACCGAGCGCAGCGA-3' was cloned into pX330
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namegRNA for VKG1 sequence
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gRNA/shRNA sequenceVKG1
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SpeciesSynthetic
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
VKG1-gRNA-pX330 was a gift from Shigeo Sugano (Addgene plasmid # 108671 ; http://n2t.net/addgene:108671 ; RRID:Addgene_108671) -
For your References section:
Development of versatile non-homologous end joining-based knock-in module for genome editing. Sawatsubashi S, Joko Y, Fukumoto S, Matsumoto T, Sugano SS. Sci Rep. 2018 Jan 12;8(1):593. doi: 10.1038/s41598-017-18911-9. 10.1038/s41598-017-18911-9 [pii] PubMed 29330493