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Addgene

p0142 pML2d BPV-1
(Plasmid #10866)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 10866 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pML2d
  • Backbone size w/o insert (bp) 2600
  • Vector type
    unknown

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BPV-1
  • Species
    Bovine papillomavirus
  • Insert Size (bp)
    7900
  • GenBank ID
    NC_001522

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See author's map and the materials and methods section of the referenced article for cloning details.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p0142 pML2d BPV-1 was a gift from Peter Howley (Addgene plasmid # 10866 ; http://n2t.net/addgene:10866 ; RRID:Addgene_10866)

  • For your References section:

    Transformation and replication in mouse cells of a bovine papillomavirus--pML2 plasmid vector that can be rescued in bacteria. Sarver N, Byrne JC, Howley PM. Proc Natl Acad Sci U S A. 1982 Dec . 79(23):7147-51. 10.1073/pnas.79.23.7147 PubMed 6296820
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