pCAGGS-6015nes
(Plasmid #108657)

• Purpose: A S6 Kinase biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)
• Depositing Lab: Michiyuki Matsuda
• Publication: Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.1038/s41598-018-27174-x.

Backbone

• Vector backbone: pCAGGS
• Backbone size w/o insert (bp): 4739
• Total vector size (bp): 7939
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: hyBRET-S6K-EV
• Species: S. cerevisiae (budding yeast), Synthetic; aequorea coerulescens
• Insert Size (bp): 3200
• Promoter: pCAGGS
• Tag / Fusion Protein:
  • XFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: pCAGGSseq_F
• 3′ sequencing primer: pCAGGSseq_R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry