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Addgene

pCAGGS-6015nes
(Plasmid #108657)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 108657 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCAGGS
  • Backbone size w/o insert (bp) 4739
  • Total vector size (bp) 7939
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    hyBRET-S6K-EV
  • Species
    S. cerevisiae (budding yeast), Synthetic; aequorea coerulescens
  • Insert Size (bp)
    3200
  • Promoter pCAGGS
  • Tag / Fusion Protein
    • XFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer pCAGGSseq_F
  • 3′ sequencing primer pCAGGSseq_R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAGGS-6015nes was a gift from Michiyuki Matsuda (Addgene plasmid # 108657 ; http://n2t.net/addgene:108657 ; RRID:Addgene_108657)
  • For your References section:

    A platform of BRET-FRET hybrid biosensors for optogenetics, chemical screening, and in vivo imaging. Komatsu N, Terai K, Imanishi A, Kamioka Y, Sumiyama K, Jin T, Okada Y, Nagai T, Matsuda M. Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.1038/s41598-018-27174-x. 10.1038/s41598-018-27174-x PubMed 29895862