pCSIIbsr-6013x
(Plasmid
#108654)
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PurposeAn HRas biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 108654 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCSIIbsr
- Backbone size w/o insert (bp) 10000
- Total vector size (bp) 13663
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Vector typeMammalian Expression, Lentiviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRaichu-EV
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SpeciesS. cerevisiae (budding yeast), Synthetic; aequorea coerulescens
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Insert Size (bp)2300
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GenBank ID
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Tag
/ Fusion Protein
- XFP (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCSIIbsr-6013x was a gift from Michiyuki Matsuda (Addgene plasmid # 108654 ; http://n2t.net/addgene:108654 ; RRID:Addgene_108654) -
For your References section:
A platform of BRET-FRET hybrid biosensors for optogenetics, chemical screening, and in vivo imaging. Komatsu N, Terai K, Imanishi A, Kamioka Y, Sumiyama K, Jin T, Okada Y, Nagai T, Matsuda M. Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.1038/s41598-018-27174-x. 10.1038/s41598-018-27174-x PubMed 29895862