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Addgene

p3998 pGEX HHR23A
(Plasmid #10864)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 10864 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-4T2
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 4970
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HHR23A
  • Species
    H. sapiens (human)
  • Entrez Gene
    RAD23A (a.k.a. HHR23A, HR23A)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer pGEX5'
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See author's map.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3998 pGEX HHR23A was a gift from Peter Howley (Addgene plasmid # 10864 ; http://n2t.net/addgene:10864 ; RRID:Addgene_10864)

  • For your References section:

    Identification of HHR23A as a substrate for E6-associated protein-mediated ubiquitination. Kumar S, Talis AL, Howley PM. J Biol Chem. 1999 Jun 25. 274(26):18785-92. 10.1074/jbc.274.26.18785 PubMed 10373495
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