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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 10861 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX-2TK
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Backbone manufacturerAmersham
- Backbone size w/o insert (bp) 4969
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameubiquitin
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Alt nameUB14
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SpeciesS. cerevisiae (budding yeast)
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Entrez GeneUBI4 (a.k.a. YLL039C, SCD2, UB14)
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Tag
/ Fusion Protein
- GST (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pGEX5' (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
See author's map.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p4027 GST-ubiquitin was a gift from Peter Howley (Addgene plasmid # 10861 ; http://n2t.net/addgene:10861 ; RRID:Addgene_10861) -
For your References section:
The HPV-16 E6 and E6-AP complex functions as a ubiquitin-protein ligase in the ubiquitination of p53. Scheffner M, Huibregtse JM, Vierstra RD, Howley PM. Cell. 1993 Nov 5. 75(3):495-505. 10.1016/0092-8674(93)90384-3 PubMed 8221889
Map uploaded by the depositor.
