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Addgene

GST PTBP1 isoform 4
(Plasmid #108594)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 108594 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX4T1
  • Backbone manufacturer
    GE Healthcare Life Sciences
  • Backbone size w/o insert (bp) 4969
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PTBP1 isoform 4
  • Alt name
    hnRNP1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1680
  • Mutation
    isoform 4 (isoform a)
  • Entrez Gene
    PTBP1 (a.k.a. HNRNP-I, HNRNPI, HNRPI, PTB, PTB-1, PTB-T, PTB2, PTB3, PTB4, pPTB)
  • Promoter tac
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer GGGCTGGCAAGCCACGTTTGGTG
  • 3′ sequencing primer CCGGGAGCTGCATGTGTCAGAGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For expression of recombinant protein use E.coli BL21. GST tag can be cleaved with Thrombin

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GST PTBP1 isoform 4 was a gift from Chris Smith (Addgene plasmid # 108594 ; http://n2t.net/addgene:108594 ; RRID:Addgene_108594)

  • For your References section:

    Nuclear matrix protein Matrin3 regulates alternative splicing and forms overlapping regulatory networks with PTB. Coelho MB, Attig J, Bellora N, Konig J, Hallegger M, Kayikci M, Eyras E, Ule J, Smith CW. EMBO J. 2015 Mar 4;34(5):653-68. doi: 10.15252/embj.201489852. Epub 2015 Jan 19. 10.15252/embj.201489852 PubMed 25599992
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