p4020 pGEM HPV-18 E6
(Plasmid
#10856)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 10856 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEM1
- Backbone size w/o insert (bp) 2865
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHPV18 E6
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Alt nameHpV18gp1
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SpeciesHuman papillomavirus
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Insert Size (bp)500
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Entrez GeneE6 (a.k.a. HpV18gp1)
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Tag
/ Fusion Protein
- HA? (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SacI? (not destroyed)
- 3′ cloning site HindIII? (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Does not translate well in Promega's TNT coupled reticulocyte lysate system. Cloning information based on data from analogous plasmids in this article.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p4020 pGEM HPV-18 E6 was a gift from Peter Howley (Addgene plasmid # 10856 ; http://n2t.net/addgene:10856 ; RRID:Addgene_10856) -
For your References section:
Association of human papillomavirus types 16 and 18 E6 proteins with p53. Werness BA, Levine AJ, Howley PM. Science. 1990 Apr 6. 248(4951):76-9. 10.1126/science.2157286 PubMed 2157286