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Addgene

p3524 pGEM HPV-16 E6
(Plasmid #10855)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 10855 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGEM1
  • Backbone size w/o insert (bp) 2865
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HPV16 E6
  • Species
    Human papillomavirus
  • Insert Size (bp)
    500
  • Entrez Gene
    E6 (a.k.a. HpV16gp1)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See author's map. Does not translate well in Promega's TNT coupled reticulocyte lysate system.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3524 pGEM HPV-16 E6 was a gift from Peter Howley (Addgene plasmid # 10855)

  • For your References section:

    Association of human papillomavirus types 16 and 18 E6 proteins with p53. Werness BA, Levine AJ, Howley PM. Science. 1990 Apr 6. 248(4951):76-9. 10.1126/science.2157286 PubMed 2157286
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