Skip to main content
Addgene

p2207 pGEM p53
(Plasmid #10853)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 10853 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pGEM4
  • Backbone size w/o insert (bp) 2870
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p53
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1200
  • GenBank ID
  • Entrez Gene
    TP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See author's map. Good for in vitro expression.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p2207 pGEM p53 was a gift from Peter Howley (Addgene plasmid # 10853 ; http://n2t.net/addgene:10853 ; RRID:Addgene_10853)

  • For your References section:

    Association of human papillomavirus types 16 and 18 E6 proteins with p53. Werness BA, Levine AJ, Howley PM. Science. 1990 Apr 6. 248(4951):76-9. 10.1126/science.2157286 PubMed 2157286
Related items:
Commonly requested with: