Skip to main content
Addgene

pCR3-vsv-GCN4-dCEN-INCENP
(Plasmid #108510)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 108510 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCR3
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GCN4-dCEN-INCENP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2724
  • Mutation
    replace CEN box for GCN4 dimerazation domain
  • Entrez Gene
    INCENP
  • Promoter CMV
  • Tag / Fusion Protein
    • VSV (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (unknown if destroyed)
  • 3′ cloning site EcoR1 (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCR3-vsv-GCN4-dCEN-INCENP was a gift from Susanne Lens (Addgene plasmid # 108510 ; http://n2t.net/addgene:108510 ; RRID:Addgene_108510)

  • For your References section:

    Inter-domain Cooperation in INCENP Promotes Aurora B Relocation from Centromeres to Microtubules. van der Horst A, Vromans MJ, Bouwman K, van der Waal MS, Hadders MA, Lens SM. Cell Rep. 2015 Jul 21;12(3):380-7. doi: 10.1016/j.celrep.2015.06.038. Epub 2015 Jul 9. 10.1016/j.celrep.2015.06.038 PubMed 26166576
Related items: