pcDNA5-FRT-TO-vsv-Incenp 8KQ-GFP
(Plasmid
#108506)
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Purposeinducible expression of vsv-INCENP 8KQ-EGFP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 108506 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA5-FRT
- Backbone size w/o insert (bp) 5100
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameINCENP
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SpeciesH. sapiens (human)
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Insert Size (bp)2757
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MutationK548Q, K563Q, K602Q, K609Q, K614Q, K622Q, K630Q, K631Q
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Entrez GeneINCENP
- Promoter CMV
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Tags
/ Fusion Proteins
- VSV (N terminal on insert)
- EGFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Age1 (unknown if destroyed)
- 3′ cloning site Age1 (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Compared to the NCBI reference sequence NM_001040694, this plasmid contains small, in-frame insertions in INCENP after aa 543 and aa 746. These insertions are extra restriction sites for cloning.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA5-FRT-TO-vsv-Incenp 8KQ-GFP was a gift from Susanne Lens (Addgene plasmid # 108506 ; http://n2t.net/addgene:108506 ; RRID:Addgene_108506) -
For your References section:
Inter-domain Cooperation in INCENP Promotes Aurora B Relocation from Centromeres to Microtubules. van der Horst A, Vromans MJ, Bouwman K, van der Waal MS, Hadders MA, Lens SM. Cell Rep. 2015 Jul 21;12(3):380-7. doi: 10.1016/j.celrep.2015.06.038. Epub 2015 Jul 9. 10.1016/j.celrep.2015.06.038 PubMed 26166576