pcDNA5-FRT-TO-vsv-Incenp wt-GFP
(Plasmid
#108502)
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Purposeinducible expression of vsv-INCENP wt-EGFP
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 108502 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA5-FRT
- Backbone size w/o insert (bp) 5100
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameINCENP
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SpeciesH. sapiens (human)
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Insert Size (bp)2757
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Entrez GeneINCENP
- Promoter CMV
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Tags
/ Fusion Proteins
- VSV (N terminal on insert)
- EGFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (destroyed during cloning)
- 3′ cloning site Not1 (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA5-FRT-TO-vsv-Incenp wt-GFP was a gift from Susanne Lens (Addgene plasmid # 108502 ; http://n2t.net/addgene:108502 ; RRID:Addgene_108502) -
For your References section:
Inter-domain Cooperation in INCENP Promotes Aurora B Relocation from Centromeres to Microtubules. van der Horst A, Vromans MJ, Bouwman K, van der Waal MS, Hadders MA, Lens SM. Cell Rep. 2015 Jul 21;12(3):380-7. doi: 10.1016/j.celrep.2015.06.038. Epub 2015 Jul 9. 10.1016/j.celrep.2015.06.038 PubMed 26166576
