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pCR3-vsv-Incenp 8KQ
(Plasmid #108501)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 108501 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR3
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    INCENP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2757
  • Mutation
    K548Q, K563Q, K602Q, K609Q, K614Q, K622Q, K630Q, K631Q
  • Entrez Gene
    INCENP
  • Promoter CMV
  • Tag / Fusion Protein
    • VSV (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Compared to the NCBI reference sequence NM_001040694, this plasmid contains small, in-frame insertions in INCENP after aa 543 and aa 746. These insertions are extra restriction sites for cloning.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCR3-vsv-Incenp 8KQ was a gift from Susanne Lens (Addgene plasmid # 108501 ; http://n2t.net/addgene:108501 ; RRID:Addgene_108501)

  • For your References section:

    Inter-domain Cooperation in INCENP Promotes Aurora B Relocation from Centromeres to Microtubules. van der Horst A, Vromans MJ, Bouwman K, van der Waal MS, Hadders MA, Lens SM. Cell Rep. 2015 Jul 21;12(3):380-7. doi: 10.1016/j.celrep.2015.06.038. Epub 2015 Jul 9. 10.1016/j.celrep.2015.06.038 PubMed 26166576
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