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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 10850 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX
- Backbone size w/o insert (bp) 5000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHPV18 E6
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Alt nameHpV18gp1
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SpeciesHuman papillomavirus
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Insert Size (bp)500
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Entrez GeneE6 (a.k.a. HpV18gp1)
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (destroyed during cloning)
- 5′ sequencing primer pGEX-5' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
See author's map. Please note this differs from the pGEX-2T sequence as indicated.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p3035 GST-HPV-18 E6 was a gift from Peter Howley (Addgene plasmid # 10850 ; http://n2t.net/addgene:10850 ; RRID:Addgene_10850) -
For your References section:
A cellular protein mediates association of p53 with the E6 oncoprotein of human papillomavirus types 16 or 18. Huibregtse JM, Scheffner M, Howley PM. EMBO J. 1991 Dec . 10(13):4129-35. PubMed 1661671
Map uploaded by the depositor.
