GFP-AuroraB kd K106R
(Plasmid #108493)

• Purpose: expression of EGFP-AuroraB kinase dead (K106R)
• Depositing Lab: Susanne Lens
• Publication: Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-59. doi: 10.1074/mcp.M111.013912. Epub 2012 Jan 20.

Backbone

• Vector backbone: pEGFP-C2
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AuroraB
• Species: H. sapiens (human)
• Insert Size (bp): 1035
• Mutation: Kinase death K106A
• Entrez Gene: AURKB (a.k.a. AIK2, AIM-1, AIM1, ARK-2, ARK2, AurB, IPL1, PPP1R48, STK-1, STK12, STK5, aurkb-sv1, aurkb-sv2)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (unknown if destroyed)
• 3′ cloning site: EcoR1 (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • SL-491 EGFP-AuroraB KD (K106A).gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene's sequencing results found M298T in AuroraB compared to the NCBI reference. The depositing lab states that there is a SNP at this location, and the discrepancy should not affect plasmid function.