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pCR3-vsv-CenpB 1-166-dCEN-Incenp-GFP
(Plasmid #108486)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 108486 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR3
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CenpB-dCEN-INCENP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3126
  • Mutation
    CEN box replaced by CenpB 1-166
  • Entrez Gene
    INCENP
  • Promoter CMV
  • Tags / Fusion Proteins
    • VSV (N terminal on insert)
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site HinDIII (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCR3-vsv-CenpB 1-166-dCEN-Incenp-GFP was a gift from Susanne Lens (Addgene plasmid # 108486 ; http://n2t.net/addgene:108486 ; RRID:Addgene_108486)

  • For your References section:

    Sensing chromosome bi-orientation by spatial separation of aurora B kinase from kinetochore substrates. Liu D, Vader G, Vromans MJ, Lampson MA, Lens SM. Science. 2009 Mar 6;323(5919):1350-3. doi: 10.1126/science.1167000. Epub 2009 Jan 15. 10.1126/science.1167000 PubMed 19150808
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