pcDNA4-Tet-on-vsv-Mis12-dCEN-Incenp-GFP
(Plasmid
#108485)
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Purposeinducible expression of vsv-Mis12-dCEN-INCENP-EGFP
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 108485 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA4-TO
- Backbone size w/o insert (bp) 5100
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Vector typeMammalian Expression
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMis12-dCEN-INCENP
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SpeciesH. sapiens (human)
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Insert Size (bp)3228
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MutationCEN box replaced by Mis12
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Entrez GeneINCENP
- Promoter CMV
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Tags
/ Fusion Proteins
- VSV (N terminal on insert)
- EGFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamH1 (destroyed during cloning)
- 3′ cloning site Xba1 (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA4-Tet-on-vsv-Mis12-dCEN-Incenp-GFP was a gift from Susanne Lens (Addgene plasmid # 108485 ; http://n2t.net/addgene:108485 ; RRID:Addgene_108485) -
For your References section:
Sensing chromosome bi-orientation by spatial separation of aurora B kinase from kinetochore substrates. Liu D, Vader G, Vromans MJ, Lampson MA, Lens SM. Science. 2009 Mar 6;323(5919):1350-3. doi: 10.1126/science.1167000. Epub 2009 Jan 15. 10.1126/science.1167000 PubMed 19150808
