pcDNA4-Tet-on-vsv-Mis12-dCEN Incenp
(Plasmid #108482)

• Purpose: inducible expression of vsv-Mis12-dCEN-INCENP
• Depositing Lab: Susanne Lens
• Publication: Liu et al Science. 2009 Mar 6;323(5919):1350-3. doi: 10.1126/science.1167000. Epub 2009 Jan 15.

Backbone

• Vector backbone: pcDNA4-TO
• Backbone size w/o insert (bp): 5100
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Mis12-dCEN-INCENP
• Species: H. sapiens (human)
• Insert Size (bp): 3228
• Mutation: CEN box replaced by Mis12
• Entrez Gene: INCENP
• Promoter: CMV
• Tag / Fusion Protein:
  • VSV (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (unknown if destroyed)
• 3′ cloning site: EcoR1 (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene's sequencing results found that translation of INCENP continues through the 3' MCS of this plasmid, resulting in an extra 55 amino acids. The depositing lab states that this change is not known to affect plasmid function.