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pCR3-vsv-dCEN-Incenp
(Plasmid #108479)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 108479 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR3
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCEN-INCENP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2622
  • Mutation
    CEN box removed
  • Entrez Gene
    INCENP
  • Promoter CMV
  • Tag / Fusion Protein
    • VSV (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCR3-vsv-dCEN-Incenp was a gift from Susanne Lens (Addgene plasmid # 108479 ; http://n2t.net/addgene:108479 ; RRID:Addgene_108479)

  • For your References section:

    The chromosomal passenger complex controls spindle checkpoint function independent from its role in correcting microtubule kinetochore interactions. Vader G, Cruijsen CW, van Harn T, Vromans MJ, Medema RH, Lens SM. Mol Biol Cell. 2007 Nov;18(11):4553-64. doi: 10.1091/mbc.E07-04-0328. Epub 2007 Aug 15. 10.1091/mbc.e07-04-0328 PubMed 17699588
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