pCR3-vsv-INCENP d543-746
(Plasmid
#108473)
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Purposeexpression of vsv-INCENP d543-746
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 108473 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCR3
- Backbone size w/o insert (bp) 5000
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameINCENP d543-746 AA
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SpeciesH. sapiens (human)
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Insert Size (bp)2151
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MutationDelta Alpha helix, aa543-746 deleted
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Entrez GeneINCENP
- Promoter CMV
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Tag
/ Fusion Protein
- VSV (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCR3-vsv-INCENP d543-746 was a gift from Susanne Lens (Addgene plasmid # 108473 ; http://n2t.net/addgene:108473 ; RRID:Addgene_108473) -
For your References section:
The chromosomal passenger complex controls spindle checkpoint function independent from its role in correcting microtubule kinetochore interactions. Vader G, Cruijsen CW, van Harn T, Vromans MJ, Medema RH, Lens SM. Mol Biol Cell. 2007 Nov;18(11):4553-64. doi: 10.1091/mbc.E07-04-0328. Epub 2007 Aug 15. 10.1091/mbc.e07-04-0328 PubMed 17699588