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Addgene

p1153 BPV-1 E2-TR
(Plasmid #10847)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 10847 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDVL
  • Backbone size w/o insert (bp) 3220
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BPV-1
  • Alt name
    E2-TR, E3, E4, E5, L2
  • Species
    Bovine papillomavirus
  • Insert Size (bp)
    1189

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    cDNA CW5-07 from Carl Baker's wart library

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See author's map.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p1153 BPV-1 E2-TR was a gift from Peter Howley (Addgene plasmid # 10847 ; http://n2t.net/addgene:10847 ; RRID:Addgene_10847)
  • For your References section:

    A transcriptional repressor encoded by BPV-1 shares a common carboxy-terminal domain with the E2 transactivator. Lambert PF, Spalholz BA, Howley PM. Cell. 1987 Jul 3. 50(1):69-78. 10.1016/0092-8674(87)90663-5 PubMed 3036366