p1153 BPV-1 E2-TR
(Plasmid
#10847)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 10847 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDVL
- Backbone size w/o insert (bp) 3220
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBPV-1
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Alt nameE2-TR, E3, E4, E5, L2
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SpeciesBovine papillomavirus
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Insert Size (bp)1189
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bycDNA CW5-07 from Carl Baker's wart library
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
See author's map.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p1153 BPV-1 E2-TR was a gift from Peter Howley (Addgene plasmid # 10847 ; http://n2t.net/addgene:10847 ; RRID:Addgene_10847) -
For your References section:
A transcriptional repressor encoded by BPV-1 shares a common carboxy-terminal domain with the E2 transactivator. Lambert PF, Spalholz BA, Howley PM. Cell. 1987 Jul 3. 50(1):69-78. 10.1016/0092-8674(87)90663-5 PubMed 3036366