p3187 HPV-16 E2
(Plasmid #10846)

• Depositing Lab: Peter Howley
• Publication: Yasugi et al J Virol. 1997 Feb . 71(2):891-9.

Backbone

• Vector backbone: pGEX-4T1
• Backbone manufacturer: Amersham
• Backbone size w/o insert (bp): 4969
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HPV-16 E2
• Alt name: E2
• Species: Human papillomavirus
• GenBank ID: NP_041328
• Entrez Gene: E2 (a.k.a. HpV16gp4)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI* (not destroyed)
• 3′ cloning site: Possibly EcoRI (not destroyed)
• 5′ sequencing primer: pGEX5'

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

HPV-16 E2 (aa1-365) from pJG4-5-derived plasmid. See author's map.

*Note that Addgene's sequence and sequence we have received from requesting labs suggest that the cloning strategy described in the depositor map is not accurate. There is a BamHI site at the 5' of the insert and an EcoRI site at the 3' end.