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PurposeThis plasmid can be used to recombine the loxP-myctagBFP-STOP-loxP-mCherry-NTR cassette (or only mCherry-NTR) into a BAC
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 108456 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC18
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Vector typeCre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsAlso Kanamycin resistance
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameloxP-myctagBFP-STOP-loxP-mCherry-NTR
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
loxP-myctagBFP-STOP-loxP-mCherry-NTR was a gift from Nadia Mercader Huber (Addgene plasmid # 108456 ; http://n2t.net/addgene:108456 ; RRID:Addgene_108456) -
For your References section:
Tbx5a lineage tracing shows cardiomyocyte plasticity during zebrafish heart regeneration. Sanchez-Iranzo H, Galardi-Castilla M, Minguillon C, Sanz-Morejon A, Gonzalez-Rosa JM, Felker A, Ernst A, Guzman-Martinez G, Mosimann C, Mercader N. Nat Commun. 2018 Jan 30;9(1):428. doi: 10.1038/s41467-017-02650-6. 10.1038/s41467-017-02650-6 [pii] PubMed 29382818