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PurposeThis plasmid can be used to recombine the mCherry-p2a-CreERT2-pA cassette (or only CreERT2-pA) into a BAC. Also to produce mCherry-p2A-CreERT2 mRNA using SP6.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 108454 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC18
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Vector typeCre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsAlso resistance to Kanamycin
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemCherry-p2a-CreERT2
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mCherry-p2A-CreERT2-FRT-kan-FRT was a gift from Nadia Mercader Huber (Addgene plasmid # 108454 ; http://n2t.net/addgene:108454 ; RRID:Addgene_108454) -
For your References section:
Tbx5a lineage tracing shows cardiomyocyte plasticity during zebrafish heart regeneration. Sanchez-Iranzo H, Galardi-Castilla M, Minguillon C, Sanz-Morejon A, Gonzalez-Rosa JM, Felker A, Ernst A, Guzman-Martinez G, Mosimann C, Mercader N. Nat Commun. 2018 Jan 30;9(1):428. doi: 10.1038/s41467-017-02650-6. 10.1038/s41467-017-02650-6 [pii] PubMed 29382818
Map uploaded by the depositor.
