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prp8-G736E
(Plasmid #108450)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 108450 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS313
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PRP8
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    8000
  • Mutation
    changed G736 to E
  • GenBank ID
    Z24732.1
  • Entrez Gene
    PRP8 (a.k.a. YHR165C, DBF3, DNA39, RNA8, SLT21, USA2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer ATG AGT GGA CTA CCG CCC C
  • 3′ sequencing primer TCA GCT AAA TAC ATC GAT TTG TTC GGC C
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    prp8-G736E was a gift from Christine Guthrie (Addgene plasmid # 108450 ; http://n2t.net/addgene:108450 ; RRID:Addgene_108450)

  • For your References section:

    Prp8 impacts cryptic but not alternative splicing frequency. Mayerle M, Yitiz S, Soulette C, Rogel LE, Ramirez A, Ragle JM, Katzman S, Guthrie C, Zahler AM. Proc Natl Acad Sci U S A. 2019 Feb 5;116(6):2193-2199. doi: 10.1073/pnas.1819020116. Epub 2019 Jan 23. 10.1073/pnas.1819020116 PubMed 30674666
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