pTRE-GFP-NMHC II-B
(Plasmid #10845)

• Depositing Lab: Robert Adelstein
• Publication: Wei et al Mol Biol Cell. 2000 Oct . 11(10):3617-27.

Backbone

• Vector backbone: pEGFP-C3
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NMHC II-B
• Alt name: nonmuscle myosin II-B heavy chain
• Species: H. sapiens (human)
• Insert Size (bp): 7400
• Entrez Gene: MYH10 (a.k.a. NMMHC-IIB, NMMHCB)
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SacII (not destroyed)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: SV40pA-R

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The CMV promoter in pEGFP-C3 is replaced by a tet-responsive promoter amplified from pTRE (Clontech).