Rich-pT7GG
(Plasmid
#108443)
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Purpose(Empty Backbone) This plasmid is useful for in vitro transcription of genes using T7 promoter. Can be used for Xenopus oocyte assay. It is derived from pT7TS-rich. The vector is compatible for Golden-gate assembly
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 108443 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneRich-pT7GG
- Backbone size (bp) 3004
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Modifications to backbonetwo sites of Bbs1 added to facilitate the golden-gate approach
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Vector typeXenopus oocyte expression system
- Promoter T7
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7P
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The plasmid is originally derived from pT7TS obtained from Addgene
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Rich-pT7GG was a gift from Richard Bélanger (Addgene plasmid # 108443 ; http://n2t.net/addgene:108443 ; RRID:Addgene_108443)
Map uploaded by the depositor.
