pHDT1:GUS
(Plasmid
#108441)
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PurposeArabidopsis transformation, expresses HDT1 promoter/GUS fusion to study expression pattern in roots
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 108441 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBnRGW
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHDT1 promoter
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SpeciesA. thaliana (mustard weed)
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Entrez GeneHDA3 (a.k.a. AT3G44750, ATHD2A, HD2A, HDT1, HISTONE DEACETYLASE 2A, histone deacetylase 3)
- Promoter HDT1
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Tag
/ Fusion Protein
- GUS (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHDT1:GUS was a gift from Ton Bisseling (Addgene plasmid # 108441 ; http://n2t.net/addgene:108441 ; RRID:Addgene_108441) -
For your References section:
Plant-Specific Histone Deacetylases HDT1/2 Regulate GIBBERELLIN 2-OXIDASE2 Expression to Control Arabidopsis Root Meristem Cell Number. Li H, Torres-Garcia J, Latrasse D, Benhamed M, Schilderink S, Zhou W, Kulikova O, Hirt H, Bisseling T. Plant Cell. 2017 Sep;29(9):2183-2196. doi: 10.1105/tpc.17.00366. Epub 2017 Aug 30. 10.1105/tpc.17.00366 PubMed 28855334