pKM468-EGFP
(Plasmid #108434)

• Purpose: ORBIT integrating plasmid for C-terminal tagging with EGFP.
• Depositing Lab: Kenan Murphy
• Publication: Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-18. doi: 10.1128/mBio.01467-18.

Backbone

• Vector backbone: pKM446
• Backbone manufacturer: Kenan Murphy
• Backbone size w/o insert (bp): 3159
• Total vector size (bp): 3879
• Modifications to backbone: Insertion of attB-EGFP sequence
• Vector type: ORBIT integrating plasmid
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Grow in LB containing 20 ug/ml chloramphenicol.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: attB-eGFP-4xGly-TEV-Flag-6xHis
• Species: Synthetic
• Insert Size (bp): 829

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Christina Baer (the source of eGFP was pEN-GFP23).
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

ORBIT plasmid for creating EGFP fusions to target genes.

Strain should be grown in 20 ug/ml chloramphenicol for isolation of plasmid. Yields with Qiagen miniprep kits are typically in the range from 60-90 ug/ml from 5 ml culture.