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Addgene

SOST/pcDNA3.1+
(Plasmid #10842)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 10842 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1+
  • Backbone size w/o insert (bp) 5428
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SOST
  • Species
    H. sapiens (human)
  • Entrez Gene
    SOST (a.k.a. CDD, DAND6, SOST1, VBCH)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SOST/pcDNA3.1+ was a gift from Xi He (Addgene plasmid # 10842 ; http://n2t.net/addgene:10842 ; RRID:Addgene_10842)

  • For your References section:

    SOST is a ligand for LRP5/LRP6 and a Wnt signaling inhibitor. Semenov M, Tamai K, He X. J Biol Chem. 2005 Jul 22. 280(29):26770-5. 10.1074/jbc.M504308200 PubMed 15908424
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