Skip to main content
Addgene

pBAD-HisD-miRFP
(Plasmid #108410)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 108410 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pBAD-HisD
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 939
  • Total vector size (bp) 4941
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    miRFP
  • Alt name
    Monomeric near-infrared fluorescent protein
  • Species
    Synthetic
  • Insert Size (bp)
    939
  • GenBank ID
    MG250278.1
  • Promoter araB

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer pBAD-Fw
  • 3′ sequencing primer pBAD-Rv
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBAD-HisD-miRFP was a gift from Edward Boyden (Addgene plasmid # 108410 ; http://n2t.net/addgene:108410 ; RRID:Addgene_108410)

  • For your References section:

    A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters. Piatkevich KD, Jung EE, Straub C, Linghu C, Park D, Suk HJ, Hochbaum DR, Goodwin D, Pnevmatikakis E, Pak N, Kawashima T, Yang CT, Rhoades JL, Shemesh O, Asano S, Yoon YG, Freifeld L, Saulnier JL, Riegler C, Engert F, Hughes T, Drobizhev M, Szabo B, Ahrens MB, Flavell SW, Sabatini BL, Boyden ES. Nat Chem Biol. 2018 Feb 26. pii: 10.1038/s41589-018-0004-9. doi: 10.1038/s41589-018-0004-9. 10.1038/s41589-018-0004-9 PubMed 29483642
Related items:
Commonly requested with: