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pcDNA3-mPC1_4148-eGFP-3HA
(Plasmid #108396)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 108396 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    modified pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pkd1
  • Alt name
    polycystin 1
  • Alt name
    PC1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1263
  • Mutation
    variant ID rs13468401 missense variant R4237Q
  • Entrez Gene
    Pkd1 (a.k.a. PC1, mFLJ00285)
  • Promoter CMV
  • Tag / Fusion Protein
    • eGFP-3HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-mPC1_4148-eGFP-3HA was a gift from Gregory Germino (Addgene plasmid # 108396 ; http://n2t.net/addgene:108396 ; RRID:Addgene_108396)

  • For your References section:

    A cleavage product of Polycystin-1 is a mitochondrial matrix protein that affects mitochondria morphology and function when heterologously expressed. Lin CC, Kurashige M, Liu Y, Terabayashi T, Ishimoto Y, Wang T, Choudhary V, Hobbs R, Liu LK, Lee PH, Outeda P, Zhou F, Restifo NP, Watnick T, Kawano H, Horie S, Prinz W, Xu H, Menezes LF, Germino GG. Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1038/s41598-018-20856-6. 10.1038/s41598-018-20856-6 [pii] PubMed 29426897
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