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AF20-double-tag
(Plasmid #108389)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 108389 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    modified pCI
  • Backbone manufacturer
    Promega
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PKD1
  • Alt name
    polycystin 1
  • Alt name
    PC1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    14481
  • Entrez Gene
    PKD1 (a.k.a. PBP, PC1, Pc-1, TRPP1)
  • Promoter CMV
  • Tags / Fusion Proteins
    • mcherry-TEV cleavage-V5 (N terminal on insert)
    • eGFP-TEV cleavage-S15 (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note, PKD1 contains P1143A mutation compared to the NCBI reference sequence NP_001009944.2, it is unknown if this mutation affects plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AF20-double-tag was a gift from Gregory Germino (Addgene plasmid # 108389 ; http://n2t.net/addgene:108389 ; RRID:Addgene_108389)

  • For your References section:

    A cleavage product of Polycystin-1 is a mitochondrial matrix protein that affects mitochondria morphology and function when heterologously expressed. Lin CC, Kurashige M, Liu Y, Terabayashi T, Ishimoto Y, Wang T, Choudhary V, Hobbs R, Liu LK, Lee PH, Outeda P, Zhou F, Restifo NP, Watnick T, Kawano H, Horie S, Prinz W, Xu H, Menezes LF, Germino GG. Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1038/s41598-018-20856-6. 10.1038/s41598-018-20856-6 [pii] PubMed 29426897
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