pcDNA5/FRT/TO-globin_del5UTR_PTC39-xrRNA-4H
(Plasmid #108376)

• Purpose: FRT integration of beta-globin reporter with premature termination codon (PTC39); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)
• Depositing Lab: Niels Gehring
• Publication: Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.1038/ncomms13691.

Backbone

• Vector backbone: pcDNA5/FRT/TO
• Backbone manufacturer: Thermo Fisher Scientific
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PTC39 beta-globin reporter with MVE xrRNA and 4H probe binding sites
• Species: H. sapiens (human)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: CGCAAATGGGCGGTAGGCGTG
• 3′ sequencing primer: TAGAAGGCACAGTCGAGG

Resource Information

• Supplemental Documents:
  • pcDNA5_FRT_TO-globin_del5UTR_PTC39-xrRNA-4H.gbk
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please see depositor's genbank file in Supplemental Documents for full annotation of the plasmid