UAS:iGluSnfr.zf1
(Plasmid #108356)

• Purpose: UAS driven variant of iGluSnFR for zebrafish expression
• Depositing Lab: Harold Burgess
• Publication: Tabor et al Curr Biol. 2018 Aug 20;28(16):2527-2535.e8. doi: 10.1016/j.cub.2018.06.020. Epub 2018 Aug 2.

Backbone

• Vector backbone: pUC19
• Backbone size w/o insert (bp): 2700
• Total vector size (bp): 5654
• Vector type: Zebrafish expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: UAS:[GlyRa1 secretion sequence]-[iGluSnFR]
• Species: Synthetic
• Insert Size (bp): 2954
• Promoter: lac

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: M13

Resource Information

• Supplemental Documents:
  • t1_14xUAS-iGluSnf-POUT
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Cloning was via SLiCE (Seamless Ligation Cloning Extract).

Note: The right tol1 region contains a single base pair mismatch compared to the depositor's expected sequence. The depositor has noted that this could reduce transgenesis efficiency, although they were able to successfully establish lines using the parent vector.