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Addgene

UAS:bloSwitch
(Plasmid #108355)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 108355 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Backbone size w/o insert (bp) 2700
  • Total vector size (bp) 6639
  • Vector type
    Zebrafish expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    UAS:[Ubiquitin-C-intron]-[blown-out site]-nuclear-GFP-[beta-globin-polyA]-[blown out site]-[membrane TagRFPT]
  • Species
    Synthetic
  • Mutation
    Zebrafish codon optimized
  • Promoter lac

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cloning was via SLiCE (Seamless Ligation Cloning Extract).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    UAS:bloSwitch was a gift from Harold Burgess (Addgene plasmid # 108355 ; http://n2t.net/addgene:108355 ; RRID:Addgene_108355)

  • For your References section:

    Presynaptic Inhibition Selectively Gates Auditory Transmission to the Brainstem Startle Circuit. Tabor KM, Smith TS, Brown M, Bergeron SA, Briggman KL, Burgess HA. Curr Biol. 2018 Aug 20;28(16):2527-2535.e8. doi: 10.1016/j.cub.2018.06.020. Epub 2018 Aug 2. 10.1016/j.cub.2018.06.020 PubMed 30078569
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