B3rec.zf1
(Plasmid
#108354)
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PurposeZebrafish codon optimized B3 recombinase that acts on blown-out sites
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 108354 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC57
- Backbone size w/o insert (bp) 2720
- Total vector size (bp) 4552
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameB3 recombinase
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SpeciesSynthetic; Zygosaccharomyces bisporus
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Insert Size (bp)1830
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MutationZebrafish codon optimized
- Promoter lac
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
B3rec.zf1 was a gift from Harold Burgess (Addgene plasmid # 108354 ; http://n2t.net/addgene:108354 ; RRID:Addgene_108354) -
For your References section:
Presynaptic Inhibition Selectively Gates Auditory Transmission to the Brainstem Startle Circuit. Tabor KM, Smith TS, Brown M, Bergeron SA, Briggman KL, Burgess HA. Curr Biol. 2018 Aug 20;28(16):2527-2535.e8. doi: 10.1016/j.cub.2018.06.020. Epub 2018 Aug 2. 10.1016/j.cub.2018.06.020 PubMed 30078569
