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Addgene

pT4/HB
(Plasmid #108352)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 108352 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript
  • Backbone manufacturer
    Stratagene
  • Backbone size (bp) 3457
  • Modifications to backbone
    Sleeping Beauty terminal inverted repeats inserted with MCS.
  • Vector type
    Transposon

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Julian Clauß, Max-Delbrück-Center for Molecular Medicine, Berlin, Germany
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The Sleeping Beauty transposon system consists of two components: the transposon vector and the transposase vector. The transposon vector (pT4/HB, Addgene #108352) contains binding sites flanking the GOI. And the transposase vector (pCMV(CAT)T7-SB100, Addgene #34879) encodes for the enzyme mediating excision and integration of the GOI.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT4/HB was a gift from Wolfgang Uckert (Addgene plasmid # 108352 ; http://n2t.net/addgene:108352 ; RRID:Addgene_108352)

  • For your References section:

    Regulated complex assembly safeguards the fidelity of Sleeping Beauty transposition. Wang Y, Pryputniewicz-Dobrinska D, Nagy EE, Kaufman CD, Singh M, Yant S, Wang J, Dalda A, Kay MA, Ivics Z, Izsvak Z. Nucleic Acids Res. 2017 Jan 9;45(1):311-326. doi: 10.1093/nar/gkw1164. Epub 2016 Dec 1. 10.1093/nar/gkw1164 PubMed 27913727
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