pML2(−)WR
(Plasmid
#108339)
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Purpose(Empty Backbone) MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 108339 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC19
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Modifications to backboneReplacement of ampicillin resistance marker with kanamycin resistance gene and f1 ori from pET-28a(+). Introduction of pheS (T251A/A294G) negative selection marker.
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Vector typeSynthetic Biology
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Selectable markersKanamycin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pML2(−)WR was a gift from Andrew Muscroft-Taylor (Addgene plasmid # 108339 ; http://n2t.net/addgene:108339 ; RRID:Addgene_108339) -
For your References section:
MIDAS: A Modular DNA Assembly System for Synthetic Biology. van Dolleweerd CJ, Kessans SA, Van de Bittner KC, Bustamante LY, Bundela R, Scott B, Nicholson MJ, Parker EJ. ACS Synth Biol. 2018 Apr 20;7(4):1018-1029. doi: 10.1021/acssynbio.7b00363. Epub 2018 Apr 12. 10.1021/acssynbio.7b00363 PubMed 29620866
Map uploaded by the depositor.
