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pSN444
(Plasmid #108331)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 108331 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    delta pSM
  • Backbone manufacturer
    C.I. Bargmann and S. MaCarroll
  • Backbone size w/o insert (bp) 3500
  • Total vector size (bp) 4688
  • Modifications to backbone
    rps-27 promoter was inserted between SphI/AscI sites.
  • Vector type
    Worm Expression
  • Selectable markers
    Nourseothricin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Streptothricin acetyl transferase 2
  • Alt name
    Sat2
  • Species
    C. elegans (nematode), Synthetic
  • Insert Size (bp)
    1358
  • Promoter rps-27 promoter

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSN444 was a gift from Shinsuke Niwa (Addgene plasmid # 108331 ; http://n2t.net/addgene:108331 ; RRID:Addgene_108331)

  • For your References section:

    Streptothricin acetyl transferase 2 (Sat2): A dominant selection marker for Caenorhabditis elegans genome editing. Obinata H, Sugimoto A, Niwa S. PLoS One. 2018 May 9;13(5):e0197128. doi: 10.1371/journal.pone.0197128. eCollection 2018. 10.1371/journal.pone.0197128 PubMed 29742140
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