pSN444
(Plasmid #108331)

• Purpose: Expresses Sat2 for Nourseothricin resistance selection of C. elegans
• Depositing Lab: Shinsuke Niwa
• Publication: Obinata et al PLoS One. 2018 May 9;13(5):e0197128. doi: 10.1371/journal.pone.0197128. eCollection 2018.

Backbone

• Vector backbone: delta pSM
• Backbone manufacturer: C.I. Bargmann and S. MaCarroll
• Backbone size w/o insert (bp): 3500
• Total vector size (bp): 4688
• Modifications to backbone: rps-27 promoter was inserted between SphI/AscI sites.
• Vector type: Worm Expression
• Selectable markers: Nourseothricin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Streptothricin acetyl transferase 2
• Alt name: Sat2
• Species: C. elegans (nematode), Synthetic
• Insert Size (bp): 1358
• Promoter: rps-27 promoter

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: gtggggtgttgttggggtgtg

Resource Information

• Supplemental Documents:
  • pSN444.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry