pcDNA3 HA Arf1 DN T31N
(Plasmid #10833)

• Depositing Lab: Thomas Roberts
• Publication: Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5428
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Arf1 T31N
• Alt name: Arf1
• Species: H. sapiens (human)
• Insert Size (bp): 550
• Mutation: T31N. Defective in GTP binding. Dominant negative.
• Entrez Gene: ARF1 (a.k.a. PVNH8)
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: bGH PA terminator

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Richard Klausner
• Articles Citing this Plasmid:
  • 9 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry