pKM464 (deletions)
(Plasmid #108322)

• Purpose: Orbit integrating plasmid to delete target gene.
• Depositing Lab: Kenan Murphy
• Publication: Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-18. doi: 10.1128/mBio.01467-18.

Backbone

• Vector backbone: pKM435
• Backbone manufacturer: Kenan Murphy
• Total vector size (bp): 3082
• Modifications to backbone: Insertion of Bxb1 attB site
• Vector type: ORBIT integration plasmid for creating knockouts of target genes
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Bxb1 attB site
• Species: Synthetic
• Insert Size (bp): 38

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

ORBIT integrating plasmid for creating knockouts of target genes.

Strain should be grown in 20 ug/ml chloramphenicol for isolation of plasmid. Yields with Qiagen miniprep kits are typically in the range from 60-90 ug/ml from 5 ml culture.