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Addgene

p006-GFP-weak
(Plasmid #108314)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 108314 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJ401
  • Backbone manufacturer
    DNA2.0
  • Backbone size w/o insert (bp) 3976
  • Total vector size (bp) 4503
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Green fluorescent protein
  • Alt name
    GFP
  • Species
    Aquorea victoria
  • Insert Size (bp)
    717
  • Promoter pFAB4282

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer CTCGAAAATAATAAAGGGAAAATCAG
  • 3′ sequencing primer CTCAGAAGTGAAACGCCGTA
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    DNA2.0, GFP comes from another plasmid pET EDNA GFP, pFAB promoter comes from the BIOFAB collection

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p006-GFP-weak was a gift from Jaroslaw Bryk (Addgene plasmid # 108314 ; http://n2t.net/addgene:108314 ; RRID:Addgene_108314)

  • For your References section:

    UNIGEMS: plasmids and parts to facilitate teaching on assembly, gene expression control and logic in E. coli. Siddall A, Williams AA, Sanders J, Denton JA, Madden D, Schollar J, Bryk J. bioRxiv 2021.06.20.449138 10.1101/2021.06.20.449138
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